Neuroprotective Effects of Asparagus officinalis Stem Extract in Transgenic Mice Overexpressing Amyloid Precursor Protein

Figure 3

Quantification of heat shock protein (HSP27) and HSP70 in the hippocampus of APP-overexpressed mice treated with ETAS (200 mg/kg and 1000 mg/kg) and WT mice treated with ETAS (1000 mg/kg) for 1 month, respectively; RFUs: relative fluorescence units; green represents HSP27/70 expression, and blue represents DAPI. Arrows represent fluorescent green staining, indicating positive expression. Magnification is represented ×200. HSP27: APP-ETAS (1000 mg/kg) vs. APP-ETAS (200 mg/kg) and APP saline-treated mice; WT-ETAS (1000 mg/kg) vs. WT saline-treated mice; RFUs: relative fluorescence units. Four mice per group and four sections per mouse were used. HSP70: APP-ETAS (1000 mg/kg) vs. APP-ETAS (200 mg/kg); APP-ETAS (1000 mg/kg) vs. APP saline-treated mice; WT-ETAS (1000 mg/kg) vs. WT saline-treated mice. RFUs: relative fluorescence units. Four mice per group and four sections per mouse were used.