RNA-seq and In Vitro Experiments Reveal the Protective Effect of Curcumin against 5-Fluorouracil-Induced Intestinal Mucositis via IL-6/STAT3 Signaling Pathway

Figure 2

Effect of curcumin on the apoptosis and inflammation of the 5-FU-stimulated IEC-6 cells. IEC-6 cells were stimulated with 5-FU for 24 h and then further treated with curcumin at 5, 10, and 20 μmol/L for 24 h. (a) Flow cytometry assay was performed to detect the cell apoptosis of each group cells. (b) The levels of TNF-α, IL-1β, and IL-6 were detected using ELISA kits. Data were expressed as (SEM), ^▲▲ value < 0.01 vs. control group; value < 0.05 vs. 5-FU group; value < 0.01 vs. 5-FU group. 5-FU: 5-fluorouracil.

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