NFIB-Mediated lncRNA PVT1 Aggravates Laryngeal Squamous Cell Carcinoma Progression via the miR-1301-3p/MBNL1 Axis

Figure 3

lncRNA PVT1 sponges miR-1301-3p. (a, b) FD-LSC-1 (a) and TU-177 (b) cells were infected with bio-NC and bio-PVT1 probes. Relative expressions of putative miRNA targets were measured by qRT-PCR. (c) Prediction of the binding sites between lncRNA PVT1 and miR-1301-3p was shown. (d–f) Dual-luciferase assay was performed to measure the association between lncRNA PVT1 and miR-1301-3p in HEK-293T (d), FD-LSC-1 (e), and TU-177 (f) cells. (g) The expression of miR-1301-3p in LSCC cells upon lncRNA PVT1 downregulation was measured by qRT-PCR. The above experiments were performed three times. , .

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