NFIB-Mediated lncRNA PVT1 Aggravates Laryngeal Squamous Cell Carcinoma Progression via the miR-1301-3p/MBNL1 Axis

Figure 4

miR-1301-3p regulates LSCC proliferation and impacts the susceptibility of LSCC cells to NK cells. (a) FD-LSC-1 and TU-177 cells were stably infected with the NC inhibitor and miR-1301-3p inhibitor to generate miR-1301-3p knockdown cell models; transfection efficiency was measured by qRT-PCR. (b–d) Cell proliferation levels were detected using CCK-8 (b, c) and cell colony assays (d). (e) Cell apoptotic rates were detected using flow cytometry. (f, g) NK cell cytotoxicity to miR-1301-3p knockdown LSCC cells was measured by calcein release assay. (h, i) Perforin polarization assay was conducted to measure the perforin-containing NK cells, which were against miR-1301-3p knockdown LSCC cells. (j, k) Conjugation assay was performed to detect the conjugate formation between NK cells and miR-1301-3p knockdown LSCC cells. All assays were conducted three times. , .

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