NFIB-Mediated lncRNA PVT1 Aggravates Laryngeal Squamous Cell Carcinoma Progression via the miR-1301-3p/MBNL1 Axis

Figure 5

MBNL1 is a downstream target of miR-1301-3p. (a) Prediction of miR-1301-3p targets of bioinformatic analysis. (b, c) RIP assay was utilized to examine the interaction between MBNL1 and miR-1301-3p. (d) The wild-type and mutant-type binding sites between miR-1301-3p and MBNL1 were shown. (e–g) Dual-luciferase assay was performed to measure the association between MBNL1 and miR-1301-3p in HEK-293T (e), FD-LSC-1 (f), and TU-177 (g) cells. (h–j) MBNL1 expression upon miR-1301-3p overexpression was detected by qRT-PCR (h) and western blotting (i, j). The above experiments were performed at least three times. , .

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