NFIB-Mediated lncRNA PVT1 Aggravates Laryngeal Squamous Cell Carcinoma Progression via the miR-1301-3p/MBNL1 Axis

Figure 6

lncRNA PVT1 regulates LSCC progression via the miR-1301-3p/MBNL1 axis. (a) FD-LSC-1 and TU-177 cells were infected with Si-NC, Si-PVT1#1, Si-PVT1#1+LV-NC, and Si-PVT1#1+LV-MBNL1; relative expressions of MBNL1 were detected. (b–d) Cell proliferation levels were detected using CCK-8 (b, c) and cell colony assays (d). (e) Cell apoptotic rates were detected using flow cytometry. (f, g) NK cell cytotoxicity to indicated LSCC cells was measured by calcein release assay. (h, i) Perforin polarization assay was conducted to measure the perforin-containing NK cells, which were against indicated LSCC cells. (j, k) Conjugation assay was performed to detect the conjugate formation between NK cells and indicated LSCC cells. All assays were conducted three times. , , and .

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