Ion Transport Modulators Differentially Modulate Inflammatory Responses in THP-1-Derived Macrophages

Figure 1

Effects of ion transport modulators on the production of nitrites and nitrates by THP-1-derived macrophages. Macrophages were stimulated with ethanol-inactivated M. bovis BCG in the presence or absence of the test drugs over 24 hours. Nitric oxide production was then assessed by quantifying levels in the supernatant using the Griess assay. Nitric oxide production was compared between the different cultures using one-way analysis of variance (ANOVA) followed by Dunnett’s post hoc test. Data are presented as of six replicates pooled from two independent experiments. Dashed line indicates nitric oxide production in drug-free controls. indicates vs. drug-free controls. HCTZ: hydrochlorothiazide.