Luteolin Regulates the Differentiation of Regulatory T Cells and Activates IL-10-Dependent Macrophage Polarization against Acute Lung Injury

Figure 2

Treatment with luteolin increases the proportion of Tregs and the expression of Foxp3 in the CLP-induced mouse model. (a, b) Treatment with luteolin significantly increased the proportion of CD4⁺CD25⁺FOXP3⁺ Tregs in splenic mononuclear cells and PBMCs of CLP mice. CD4⁺CD25⁺FOXP3⁺ Tregs in splenic mononuclear cells and PBMCs were detected by flow cytometry. (c) Treatment with luteolin increased the expression of Foxp3 in lungs of CLP mice. Foxp3 protein levels were measured by western blot. (d) Treatment with luteolin promotes the expression of IL-10 in serum and BALF of CLP mice, thereby playing an anti-inflammatory role. IL-10 levels in serum and BALF of mice were measured using a mouse cytokine/chemokine magnetic bead panel kit. Each group , experiments are repeatable, and the most representative one was shown. Data of the column graphs are presented as . NS: not significant. , , , and by the one-way ANOVA followed by LSD multiple comparison test, compared between the control, CLP, and CLP+Lut groups.

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