Luteolin Regulates the Differentiation of Regulatory T Cells and Activates IL-10-Dependent Macrophage Polarization against Acute Lung Injury

Figure 3

Treatment with luteolin regulates M1 and M2 macrophage phenotypes in lungs of CLP mice. (a) Treatment with luteolin reduces the proportion of M1 macrophages, while increasing the proportion of M2 macrophages in lungs of CLP mice, hence playing a protective role in ALI. The proportion of pink fluorescent iNOS expressing M1 macrophages increased significantly, while lungs of CLP mice were substantially negative for green fluorescent CD206⁺ M2 macrophages. Treatment with luteolin reduced the proportion of M1 while increasing that of M2 macrophages in ALI. Five 400x fields of positive cells were counted and analyzed. Each group , experiments are repeatable, and the most representative one was shown. Data of the column graphs are presented as . by the one-way ANOVA followed by LSD multiple comparison test, compared between the control, CLP, and CLP+Lut groups.

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