Luteolin Regulates the Differentiation of Regulatory T Cells and Activates IL-10-Dependent Macrophage Polarization against Acute Lung Injury

Figure 4

Luteolin plays a protective role in ALI, which is potentially linked to IL-10 and Tregs. (a, c, d) After treatment with CD25 neutralizing antibodies, the number of Tregs in the spleen of mice in the CLP+Lut group was significantly reduced. IL-10 expression was significantly reduced, and lung injury was significantly worse. CD4⁺CD25⁺FOXP3⁺ Tregs in splenic mononuclear cells were detected by flow cytometry. Lungs from each experimental group were stained with H&E and processed for histological examination. Mouse IL-10 serum levels were measured by ELISA. (b) After treatment with CD25 neutralizing antibodies, the expression of Foxp3 in lungs of GL+CLP mice was decreased. Foxp3 protein levels were measured by western blot. Each group , experiments are repeatable, and the most representative one was shown. Data of the column graphs are presented as . and between groups represented by horizontal lines, as calculated by one-way ANOVA followed by LSD multiple comparison test.

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