Major Outer Membrane Protein from Legionella pneumophila Inhibits Phagocytosis but Enhances Chemotaxis of RAW 264.7 Macrophages by Regulating the FOXO1/Coronin-1 Axis

Figure 1

MOMP inhibits the proliferation and phagocytic activity of RAW264.7. (a) A dose-response study of MOMP indicated IC50 as 4.05 μg/ml. The curve was fitted using relative survival fraction of six working concentrations (10, 5, 2.5, 1.25, 0.625, and 0.3125 μg/ml). (b) The proliferating capacity of RAW 264.7 upon the treatment of MOMP. Statistical analysis by one-way ANOVA, . Error confidence intervals. (c) The cytological phagocytosis of RAW 264.7 by neutral red uptake assay at 24 h in the four groups (control; cells treated with 0.2 μg/ml MOMP; cells treated with 0.4 μg/ml MOMP; and cells treated with 0.8 μg/ml MOMP) (original magnification: 200x, scale ), indicated significant differences in neutral red uptake of cells between control and treated groups. (d) Semiquantitative analysis of the phagocytosis of RAW 264.7. The significant differences were marked by the alphabetic notation (a, b, c, and d). In each same time point of different concentration treatment groups, if any two histograms with the same marking letter represented there have no significant difference between two concentration treatments, any two histograms without the same marking letter represented there have a significant difference.

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