Major Outer Membrane Protein from Legionella pneumophila Inhibits Phagocytosis but Enhances Chemotaxis of RAW 264.7 Macrophages by Regulating the FOXO1/Coronin-1 Axis

Figure 3

Analysis of mRNA expression of 7 proteins in RAW 264.7 treated with MOMP. The significant differences in the expression of 7 proteins in groups treated with different concentrations were marked by the alphabetic notation (a, b, c, and d). In each detected expression of 7 proteins, if any two histograms with the same marking letter represented no significant difference between two concentrations, any two histograms without the same marking letter represented a significant difference. (a–g) Relative mRNA expression analysis of NOD1, NOD2, IL-10, RIP, MCP-1, FOXO1, and coronin-1 for 24 h treated by different concentrations of MOMP. Control represented the cells cultured in a normal medium. 0.2 represented the cells cultured in the medium containing 0.2 μg/ml MOMP, 0.4 represented the cells cultured in the medium containing 0.4 μg/ml MOMP, and 0.8 represented the cells cultured in the medium containing 0.8 μg/ml MOMP (error ).

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