Major Outer Membrane Protein from Legionella pneumophila Inhibits Phagocytosis but Enhances Chemotaxis of RAW 264.7 Macrophages by Regulating the FOXO1/Coronin-1 Axis

Figure 4

Expression analysis of 7 proteins at the protein level in RAW 264.7 treated by MOMP for 24 h. The significant differences in the expression of 7 proteins in groups treated with different concentrations were marked by the alphabetic notation (a, b, c, and d). In each detected expression of 7 proteins, if any two histograms with the same marking letter represented no significant difference between two concentration treatments, any two histograms without the same marking letter represented a significant difference. Relative protein expression analysis in each group was performed on the basis of the gray value of internal control β-actin. (a) Western blotting analysis of 7 proteins and β-actin. (b–h) Relative protein expression of FOXO1, MCP-1, coronin-1, IL-10, NOD1, NOD2, and RIP2 for 24 h treated by different concentrations of MOMP. Control represented the cells cultured in the normal medium; 0.2 represented the cells cultured in the medium containing 0.2 μg/ml MOMP; 0.4 represented the cells cultured in the medium containing 0.4 μg/ml MOMP; 0.8 represented the cells cultured in the medium containing 0.8 μg/ml MOMP (error ).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)