Maternal Immune System and State of Inflammation Dictate the Fate and Severity of Disease in Preeclampsia

Figure 3

Analysis of MMP-12, iNOS, TLR4, and HMGB-1 expressions from PPE vs. preterm controls with/out smoking history. (a) Analysis of MMP-12 proteins in the placental tissues of preterm preeclampsia compared to preterm pregnancy cohort with/out smoking history by Western blotting. MMP-12 expression data shown in the WB image is a representative of specimens isolated from 8-30 specimen samples belonging to subjected used in the study. (b, c) mRNA levels of the human MMP-12, iNOS, TLR4, and HMGB-1 transcripts were analyzed in placental tissue by real-time polymerase chain reaction (RT-PCR). 1 μg total RNA was reverse transcribed in 20 μl reaction. A total of 2 μl cDNA and the 1 μM real-time PCR primers purchased from Real Time Primers, LLC (Elkins Park, PA) were used for amplification in Bio-Rad CFX-96 Real Time System. Expression of the target genes was normalized to β-actin and displayed as fold change relative to the preterm control sample. Data are representative of specimens isolated from 8-30 specimen samples belonging to subjected used in the study. s denotes smoker, and ns denotes nonsmoker. represents statistically different with value <0.05.

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