Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα

Figure 1

I/R-induced Plin2 upregulation and AKI. Eight-week-old C57/BL6 mice were treated with I/R and euthanized after 0, 6, and 24 h. (a) Serum creatinine (SCr) levels in the different groups of mice (-5). (b) Representative terminal deoxynucleotidyl transferase–mediated dUTP nick end-labeling (TUNEL) staining of kidney sections 24 h after renal IR (). (c) Representative images of dihydroethidium (DHE) staining of reactive oxygen species (ROS) generation of kidney sections 24 h after renal IR (). (d) Costaining for Plin2 and lipid droplet in kidney sections 24 h after renal IR (). (e) Quantitative RT-PCR analysis of Plin2 mRNA expression in the different groups of mice after renal IR (). (f) Western blot analysis of Plin2 expression in the different groups of mice after renal IR (). vs. sham mice. Data are presented as . h: hour; IR: ischemia reperfusion.

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