Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα

Figure 2

Plin2 levels increase after hydrogen peroxide treatment in primary renal proximal tubular cells. Primary renal proximal tubular cells were treated with hydrogen peroxide. (a, b) Cell viability measured using CCK-8 assays after hydrogen peroxide treatment (). vs. 0 h or 0 μM hydrogen peroxide. (c) Images of mitochondrial reactive oxygen species (ROS) generation after hydrogen peroxide treatment for 12 hours. (d) Quantitative RT-PCR analysis of Plin2 mRNA expression after hydrogen peroxide treatment for 12 hours (). vs. 0 μM hydrogen peroxide. (e–h) Western blot analysis of Plin2, BAX, Bcl-2, and cleaved caspase-3 expressions after hydrogen peroxide treatment for 12 hours (). vs. 0 μM hydrogen peroxide; vs. 0 μM hydrogen peroxide; and vs. 0 μM hydrogen peroxide. Data are presented as . H₂O₂: hydrogen peroxide.

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