Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα

Figure 3

Knockdown of Plin2 attenuates apoptosis in primary renal proximal tubular cells. (a) Western blot analysis of Plin2 expression after Plin2 knockdown (). vs. the NC group. (b) Cell viability measured using CCK8 assays (). vs. the NC group, ^# vs. the NC or H₂O₂ group. Data are presented as . (c) Images of mitochondrial reactive oxygen species (ROS) generation in PTECs transfected with Plin2 knockdown plasmid after hydrogen peroxide (300 μM) treatment. (d, e) Western blot analysis of Plin2 () and cleaved caspase-3 () expression in primary renal proximal tubular cells transfected with Plin2 knockdown plasmid after hydrogen peroxide (300 μM) treatment (). vs. the NC group, vs. the NC group, ^# vs. the NC or H₂O₂ group, and ^## vs. the NC or H₂O₂ group. (f) Cell apoptosis in hydrogen peroxide (300 μM)-treated renal proximal tubular cells transfected with Plin2 knockdown plasmid or control plasmid. Annexin V-positive cells were considered apoptotic cells (). vs. the NC group, ^# vs. the H₂O₂ group, and ^## vs. the NC group. Data represent the . H₂O₂: hydrogen peroxide; NC: negative control.

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