Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα

Figure 4

Plin2 impacts apoptosis via the regulation of PPARα. (a, b) Expression of PPARα after hydrogen peroxide treatment (). vs. 0 μM hydrogen peroxide; vs. 0 μM hydrogen peroxide; and vs. 0 μM hydrogen peroxide. (c) Western blot analysis of PPARα in primary renal proximal tubular cells transfected with Plin2 knockdown plasmids after hydrogen peroxide treatment (). vs. NC;^### vs. hydrogen peroxide. (d, e) Cell viability of Plin2 knockdown or overexpression in primary renal proximal tubular cells after hydrogen peroxide treatment (). vs. DMSO NC. (f) Western blot analysis of cleaved caspase-3 expression in primary renal proximal tubular cells transfected with Plin2 overexpression plasmid with or without fenofibrate after hydrogen peroxide (300 μM) treatment (), , . (g, h) Cell apoptosis in hydrogen peroxide (300 μM)-treated renal proximal tubular cells transfected with Plin2 knockdown plasmid or control plasmid with or without fenofibrate. Annexin V-positive cells were considered apoptotic cells (), , . Data represent . H₂O₂: hydrogen peroxide; NC: negative control; OE: overexpression.

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