The Activation of the Tumor Suppressor Protein p53 by Acriflavine Leads to Mitochondrial Dysfunction and Improves the Radiosensitivity of Colon Cancer Cells

Figure 3

Overexpression of p53, mitochondrial dysfunction, and enhanced radiosensitivity of SW620 cells. (a) Overexpression of p53 was detected successfully by QRT-PCR. (b) The experimental results of QRT-PCR showed that the mRNA expression levels of Bcl-2 decreased with overexpression of p53, while the mRNA expression levels of Bad and Bax increased. (c, d) Western Blot results showed that overexpression of p53 led to a decrease in the expression level of Bcl-2, while the expressions of Bad, Bax, AIF, and cytochrome C increased. (e) MTT results showed that overexpression of p53 weakened the proliferation ability of SW620 cells. (f) The results of in vitro invasion experiment showed that the invasion ability of SW620 cells was inhibited by overexpression of p53. (g) Flow cytometry showed that apoptosis of SW620 cells was enhanced. (h) Luciferase reporting experiment results showed that there was a binding site between p53 and ACF. , compare with the control group.

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