Intratumoral IL-28B Gene Delivery Elicits Antitumor Effects by Remodeling of the Tumor Microenvironment in H22-Bearing Mice

Figure 2

Intratumoral IL-28B gene transfer decreased the frequency of Tregs in the spleen. Spleens were harvested; processed into single-cell suspension; stained with FITC-conjugated anti-CD4, PerCP-conjugated anti-CD8, PE-conjugated anti-PD-1, and APC-conjugated anti-Foxp3; and analyzed by flow cytometry. (a) Representative dot plots and percentages of cells bounded by each region are shown in panels. (b) Statistical analysis of the percentages of CD4⁺ T cells, CD8⁺ T cells, and CD4⁺Foxp3⁺ cells among CD4⁺ T cells, CD4⁺PD-1⁺ T cells, CD8⁺PD-1⁺ T cells, and CD4⁺ Foxp3⁺ PD-1⁺ T cells in the spleens were shown. Data from one experiment, each performed with three mice, are shown. , ; and ns indicated .

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