Intratumoral IL-28B Gene Delivery Elicits Antitumor Effects by Remodeling of the Tumor Microenvironment in H22-Bearing Mice

Figure 4

The peripheral and local cytokines production altered by IL-28B in vivo. Serum and tumor tissues were harvested and analyzed the cytokines by use of a commercial quantibody mouse cytokine array 5. The array images (a), a heat map of cytokine expression (b), and the mean signals strength of CXCL13, ICAM-1, MCP-5, IL-7 IFN-γ, IL-2, and TNF-α in the serum (c) were shown. The array images (d), a heat map of cytokine expression (e), and the mean signals strength of IL-15, sFasL, IFN-γ, IL-2, and TNF-α in TME (f) were shown. The mean signals strength derived from quadruplicate samples (). The hierarchical cluster analysis are used to analyze dissimilarities between serum and tumor tissues of rAd-mIL-28B (g). The moderated -statistic was used for significance analysis to screen the differential proteins, which were defined as those with adjusted and or . Red indicates higher expressions and green indicates lower expressions. The levels of cytokines which changed more than twofold in serum and tumor tissues were shown (h). The KEGG pathway analysis (i) and Gene Ontology- (GO-) based biological process annotation of the differentially expressed proteins (j) were shown. Data from one experiment, each performed with three mice, are shown. TME: tumor microenvironment. ; ns indicated .

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