Intratumoral IL-28B Gene Delivery Elicits Antitumor Effects by Remodeling of the Tumor Microenvironment in H22-Bearing Mice

Figure 5

IL-28B inhibited the proportion of Foxp3⁺-producing cells in a dose-dependent manner concomitant with a reduction in IL-10 production and Foxp3 mRNA expression. Splenocytes isolated from normal BALB/C mice were stimulated with anti-CD3/CD28 mAbs supplemented with IL-2 in the presence of TGF-β±IL-28B (5, 50, and 400 ng/ml). Splenocytes cultured in complete RPMI 1640 medium were used as the control. Cells stimulated with 500 ng/ml anti-CD3/CD28 mAbs, 5 ng/ml IL-2, and 1.25 ng/ml TGF-β were defined as the stimulation group. After three days, cells were harvested for flow cytometry and RT-PCR analysis, and cell-free culture was collected for IL-10 assay. (a) The percentages of Foxp3⁺ T cells were shown in bar graphs. Data were from six independent experiments. (b, c) The Foxp3 mRNA expression and IL-10 level were shown. Data from one experiment, each performed with three samples, are shown. ; .

(a)

(b)

(c)