29 m⁶A-RNA Methylation (Epitranscriptomic) Regulators Are Regulated in 41 Diseases including Atherosclerosis and Tumors Potentially via ROS Regulation – 102 Transcriptomic Dataset Analyses

Figure 8

The expressions of m⁶A-RNA methylation regulators (m⁶A-RMRs) were modulated in endothelial cells with different stimulations. (a) The expression changes of m⁶A-RMRs were the most obvious in influenza virus infections with the highest expression FC values. The expressions of writer KIAA1429 and readers YTHDF1, YTHDF2, IGF2BP1, IGF2BP2, HNRNPC, and ELAVL1 were upregulated with the ; the expressions of writers METTL3, METTL14, WTAP, ZC3H13, RBM5, RBM15B; eraser FTO; and readers YTHDF3, YTHDC2, HNRNPA2B1, IGF2BP3, G3BP1, and G3BP2 were downregulated with the . In KSHV-infected ECs, IGF2BP3 was downregulated with low FC values. In LPS-treated microvascular ECs, writer WTAP was upregulated in four and eight hours. In IFNa- and IFNb-treated cells, FMR1 was increased. In the cells with NOTCH1 knockdown combined with IL-1β treatment, the expression changes of m⁶A-RMRs were not obvious. ECs activated with ox-PAPC showed that the expressions of WTAP and CBLL1 were upregulated. There were gentle expression changes of m⁶A-RMRs for ECs exposed to shear or for mouse aortic ECs from apolipoprotein E- (ApoE-) deficient (ApoE knock out) or treated with LPS, oxLDL, and oxPAPC, respectively. (b) The m⁶A-RMRs were differentially expressed in aortic EC populations. The 12 out of 29 m⁶A-RMRs including Wtap, Zc3h13, Pcif1, FTO, Alkbh5, Ythdc1, YthDf2, Ythdf3, Fmr1, Prrc2a, Elavl1, and G3bp1 were in medium expression levels in almost all aortic cell populations of normal mouse aorta; and m⁶A-RMRs such as Hnrnpa2b1, Eif3a, Hnmpc, and G3bp2 were differentially expressed in aortic cell populations. (Mettl14, Igf2bp1, and Igf2bp2 not found) (). Abbreviations: KSHV: Kaposi sarcoma-associated herpes virus; oxPAPC: oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (inflammatory lipids); oxLDL: oxidized LDL; FC: fold change; NA: not available (missing value); VSMC: vascular smooth muscle cells; EC: endothelial cells.

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