NLRP3 Inflammasome Simultaneously Involved in Autophagy and Phagocytosis of THP-1 Cells to Clear Aged Erythrocytes

Figure 4

Autophagy of THP-1 caused by engulfing RBCs is inhibited by the autophagy inhibitor bafromycin. (a) Merge photograph of THP-1 cells stably expresses the mRFP-GFP-LC3 fusion protein after different stimulations. The above column shows the imaging results of the fluorescence inverted microscope (200×), whereas the below column indicates the results from the confocal microscope (1,000×). The method of image data analysis and processing is the same as that used in Figures 3(b)–3(d). (b)–(d) Histograms of red cell rates of three different THP-1 cells to the same RBCs with or without bafromycin. , , and ns: not significant.

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