NLRP3 Inflammasome Simultaneously Involved in Autophagy and Phagocytosis of THP-1 Cells to Clear Aged Erythrocytes

Figure 5

Autophagy of THP-1 caused by engulfing RBCs is inhibited by the downregulated expression of NLRP3. Three relative amounts (p62 vs. GAPDH, LC3 II vs. GAPDH, and LC3 II vs. LC3 I) were calculated according to gray values obtained in Figure 6(a). These data focus on the autophagy of wild type, shNC, and ID3 THP-1 cells after phagocytosis of the same RBCs without bafromycin. ID3 THP-1 cells showed the minimum autophagy marker regardless of the kind of RBCs they were incubated with. , , and ns: not significant.