NLRP3 Inflammasome Simultaneously Involved in Autophagy and Phagocytosis of THP-1 Cells to Clear Aged Erythrocytes

Table 3

Engulf ability of THP-1 cells to erythrocytes is inhibited using rapamycin.

(a)

Phagocytosis rates of RBCs (100%)
	Untreated RBCs			42°C-incubated RBCs			IgG-opsonized RBCs
	With rapamycin	Without rapamycin	value	With rapamycin	Without rapamycin	value	With rapamycin	Without rapamycin	value
Wild-type THP-1
shNC THP-1
ID3 THP-1

(b)

	Decreased RBC clearance rate inhibited by rapamycin (100%)			value
	Wild-type THP-1	shNC THP-1	ID3 THP-1	1	2	3
Untreated RBCs				Ns
42°C-incubated RBCs				Ns
IgG-opsonized RBCs				Ns

value, 1: Wild-type THP-1 vs. shNC THP-1; 2: Wild-type THP-1 vs. ID3 THP-1; and 3: shNC THP-1 vs. ID3 THP-1. , ns: not significant.