Associations between Circulating VEGFR2^hi-Neutrophils and Carotid Plaque Burden in Patients Aged 40-64 without Established Atherosclerotic Cardiovascular Disease

Figure 1

Gating strategy of flow cytometry. Sequential gating strategy for the identification of neutrophil subpopulations. Granulocytes were gated based on Forward Scatter (FSC) and Side Scatter (SSC) (а). Furthermore, CD16⁺ cells were identified, and single cells were gated based on FSC Time-Of-Flight and FSC Integral (c) and SSC Time-Of-Flight and SSC Integral (d). Identification of mature neutrophil subpopulations depending on CD11b and CD62L expression (CD11b^hiCD62L^hi) (e). Identification of aging neutrophils by the expression of CD184 and CD182 (f). Identification of VEGFR2^hi-neutrophils by the expression of CD309 and CD182 (g).

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