Macrophage Depletion Reduces Disease Pathology in Factor H-Dependent Immune Complex-Mediated Glomerulonephritis

Table 1

Primer sequences used to assess gene expression by qRT-PCR.

Gene	Forward primer sequence	Reverse primer sequence
GAPDH,	5-TTGATGGCAACAATCTCCAC-3	5- CGTCCCGTAGACAAAATGGT-3
Markers:
CD206	5-CAGGAGGAGAGAGATCCGATTTA-3	5- GCATTAGCATGGAAGCAAAGA-3
CD204	5 TGGAGGAGAGAATCGAAAGCA-3	5- CTGGACTGACGAAATCAAGGAA-3
Matrix proteins:
TGF-β1	5- TGCGCTTGCAGAGATTAAAA-3	5’-AGGTAACGCCAGGAATTGTTGCTA-3
MMP9	5-CTTCTGGCGTGTGAGTTTCCA-3	5-ACTGCACGGTTGAAGCAAAGA-3
Laminin,	5-CGGAGCCCTGCATCACAAA -3	5- AGCAAGGTCGTCCTCAAAGC -3

ELISA: serum TNFα and IL-6 levels were measured by ELISA MAX™ Standard Kits (BioLegend, CA, USA) according to the manufacturer’s protocols. The concentrations of IL-1B, M-CSF, and GM-CSF were measured by ELISA (R&D Systems, UK) according to the manufacturer’s instructions.