Asiaticoside Suppresses Gastric Cancer Progression and Induces Endoplasmic Reticulum Stress through the miR-635/HMGA1 Axis

Figure 2

Asiaticoside suppresses gastric cancer aggressive behaviors and induced endoplasmic reticulum stress by upregulating the miR-635 expression. (a) The miR-635 expression in GES-1, AGS, and HGC27 cells was assessed using qRT-PCR. (b) The miR-635 expression in AGS and HGC27 cells after treatment with AC was evaluated. (c) The miR-635 expression was tested utilizing qRT-PCR after cell treatment with AC and transfected with the miR-635 inhibitor. (d) Gastric cancer cell viability was assessed by the CCK-8 assay after AC treatment and miR-635 inhibitor transfection. (e) The migration capacity was evaluated by the Transwell assay after AC treatment and miR-635 inhibitor transfection. (f) The levels of PCNA, E-cadherin, and N-cadherin after AC treatment and miR-635 inhibitor transfection were determined by Western blot. (g) The levels of GRP78, Chop, and hnRNPA1 in AGS and HGC27 cells after treatment with AC and transfection with the miR-635 inhibitor were evaluated using Western blot. .

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