ADAM-10 Regulates MMP-12 during Lipopolysaccharide-Induced Inflammatory Response in Macrophages

Figure 3

Interfered A disintegrin and metalloprotease 10 (sh ADAM-10) decreases lipopolysaccharide-promoted proliferation- (LPS-) induced macrophage activation via matrix metalloproteinase 12 (MMP-12) downregulation. (a) The effect of LPS and MMP-12 inhibitors on sh ADAM-10 RAW246.7 and sh ADAM-10 J774a.1 cell viability. Each group contained six samples. (b) Real-time qualitative PCR (RT-qPCR) analysis of ADAM-10 and MMP-12 transcription levels in response to LPS and MMP-12 inhibitors in sh ADAM-10 RAW246.7 cells. Each group contained six samples. (c) Flow cytometry analysis shows inhibition of ADAM-10 decreased MMP-12 expression, while inhibition of MM-12 has no significant effect on ADAM-10.

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