ADAM-10 Regulates MMP-12 during Lipopolysaccharide-Induced Inflammatory Response in Macrophages

Figure 4

Overexpressing A disintegrin and metalloprotease 10 (EX ADAM-10) increases lipopolysaccharide-promoted proliferation- (LPS-) induced macrophage activation via matrix metalloproteinase 12 (MMP-12) upregulation. (a) Real-time qualitative PRC (RT-qPCR) analysis of the effect of EX ADAM-10 in RAW264.7 cells. Each dot represents a readout. (b) Comparison of the gene transcription levels of interfered ADAM (sh ADAM-10) and EX ADAM-10 in RAW264.7 cells by RT-qPCR. Each dot represents a readout. (c) RT-qPCR analysis of the effect of LPS on gene transcription in EX ADAM-10 RAW246.7 cells. Each dot represents a readout. (d) The effect of LPS and MMP-12 inhibitors on EX ADAM-10 RAW246.7 cell viability. Each group contained six samples. (e) Enzyme-linked immunosorbent assay (ELISA) analysis of ADAM-10 secretion in response to LPS and MMP-12 inhibitors in EX ADAM-10 RAW246.7 cells. Each group contained six samples. (f) Flow cytometric analysis showed that EX ADAM-10 upregulated MMP-12 expression. (g) Immunofluorescence of EX ADAM-10 RAW246.7 cells, followed by treatment with LPS and MMP-12 inhibitors. . Data are expressed as the . and compared with the CON group; ^# and ^## compared with the LPS group.

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