Revealing Potential Diagnostic Gene Biomarkers Associated with Immune Infiltration in Patients with Renal Fibrosis Based on Machine Learning Analysis

Figure 9

In vitro, suppression of the ISG20 gene significantly reduced the evolution of renal fibrosis. (a, b) In patients with renal fibrosis, the expression of ISG20 and SERPINA3 was found to be upregulated in a unique manner. (c, d) For 48 hours, HK-2 cells were exposed to concentrations of 2, 5, or 10 ng/ml TGF-1. qRT-PCR was used to detect the expressions of ISG20 and SERPINA3 in HK-2 cells. (e) ISG20 siRNA was transfected into HK-2 cells. Subsequently, using RT-PCR, we discovered that ISG20 was expressed in HK-2 cells. (f, g) RT-PCR was used to find out how much mRNA fibronectin and α-SMA were making in HK-2 cells. (h) The protein expressions of fibronectin and α-SMA in HK-2 cells were determined by western blot.

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