Pharmacological Inhibition of Glutaminase 1 Normalized the Metabolic State and CD4+ T Cell Response in Sjogren’s Syndrome

Figure 2

BPETS treatment normalized both glycolysis and OXPHOS in CD4+ T cells of SS-like NOD/Ltj mice. (a) The real-time OCR values in CD4+ T cells treated with 0.1 μM to 10 μM BPTES. (b) Measurement of basal oxygen consumption in CD4+ T cells treated with 0.1 μM to 10 μM BPTES. (c) Measurement of mitochondrial ATP respiration in CD4+ T cells treated with 0.1 μM to 10 μM BPTES. (d) The real-time ECAR values in CD4+ T cells treated with 0.1 μM to 10 μM BPTES. (e) Measurement of the basal glycolytic levels in CD4+ T cells treated with 0.1 μM to 10 μM BPTES. (f) Measurement of glycolytic capacity under stress in CD4+ T cells treated with 0.1 μM to 10 μM BPTES. (g) Measurement of α-KG and pyruvate concentration in CD4+ T cells treated with 0.1 μM to 10 μM BPTES. (h) Measurement of ATP concentration in CD4+ T cells treated with 0.1 μM to 10 μM BPTES. Summary data are presented as with unpaired two-tailed Student’s -tests.

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