Pharmacological Inhibition of Glutaminase 1 Normalized the Metabolic State and CD4+ T Cell Response in Sjogren’s Syndrome

Figure 3

BPTES treatment confined the proliferation of CD4+ T cells upon activation. (a) Representative flow cytometry images of the CFSE assay in activated CD4+ T cells treated with the indicated doses of BPTES. (b, c) Representative flow cytometry images and statistical analysis of the EdU assay in activated CD4+ T cells treated with different dosages of BPTES. (d) Representative Western blot pictures of Cyclin D3, Cdc25, and Cdk2 in activated CD4+ T cells treated with different doses of BPTES. (e) ImageJ quantification of the relative expression of Cyclin D3, Cdc25, and Cdk2 in activated CD4+ T cells treated with different doses of BPTES. Summary data are presented as with unpaired two-tailed Student’s -tests.

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