Pharmacological Inhibition of Glutaminase 1 Normalized the Metabolic State and CD4+ T Cell Response in Sjogren’s Syndrome

Figure 4

Treatment with BPTES alleviated the symptoms of SS and CD4+ T cell proportions in NOD/Ltj mice. (a) Simulated salivary flow rate of NOD/Ltj mice and wild-type ICR mice treated with the indicated doses of BPTES. The salivary flow rate was measured at the ages of 8 weeks, 10 weeks, and 12 weeks. (b) Pictures of H&E staining in NOD/Ltj mice treated with/without 10 mg/kg BPTES. (c) Pictures of immunohistochemical staining of CD4 in NOD/Ltj mice treated with/without 10 mg/kg BPTES. (d) Pictures of immunohistochemical staining of PCNA in NOD/Ltj mice treated with/without BPTES. (e) Representative picture of flow cytometry in salivary glands of the indicated NOD/Ltj mice. The proportions of CD4+ T cells (CD3+CD4+CD45+) and CD8+ T cells (CD3+CD8+CD45+) were gated and evaluated. (f) Measurement of the absolute CD4+ T cell numbers in NOD/Ltj mice treated with/without BPTES. (g) Measurement of the percentage of CD4+ T cells in total T cell populations in NOD/Ltj mice treated with/without BPTES. (h) Measurement of the percentage of CD8+ T cells in total T cell populations in NOD/Ltj mice treated with/without BPTES. (i) Measurement of CD4+/CD8+ T cell ratios in NOD/Ltj mice treated with/without BPTES. Summary data are presented as with unpaired two-tailed Student’s -tests.

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