Pharmacological Inhibition of Glutaminase 1 Normalized the Metabolic State and CD4+ T Cell Response in Sjogren’s Syndrome

Figure 5

BPTES treatment selectively inhibited the effector function of CD4+ T cells and the immune response in NOD/Ltj mice. (a, b) Representative pictures and statistical analysis of flow cytometry data of IFNγ-producing and IL-17A-producing cells in the CD4+ T cell population. (c) qRT–PCR analysis of IFNγ and IL-17A gene expression in NOD/Ltj mice treated with/without 10 mg/kg BPTES. (d) ELISA quantification of IFNγ and IL-17A levels in circulating sera of the indicated NOD/Ltj mice. (e) ELISA quantification of IL-2 and TNF-α levels in circulating sera of the indicated NOD/Ltj mice. (f) ELISA quantification of ANA, anti-SSA, and anti-SSB levels in circulating sera of the indicated NOD/Ltj mice. Summary data are presented as with unpaired two-tailed Student’s -tests.

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