Peptidoglycan-Like Components in Z-100, Extracted from Mycobacterium tuberculosis Strain Aoyama B, Increase IL-12p40 via NOD2

Figure 1

Cytokine production from BMDM following Z-100 stimulation. (a) Schematic of the experimental design. Bone marrow-derived cells were cultured with GM-CSF for 7 days before being seeded onto plates and preincubated for 30 minutes. After preincubation, stimulation was started. (b, c) IL-12p40 concentration in culture supernatant of BMDM after stimulation for 24 hours with Z-100 (b; 10, 30, or 100 μg/mL) or Pam3CSK4 (c; 3, 10, or 30 ng/mL). Symbols indicate a significant difference compared with the 0 μg/mL (saline) group; (Steel’s multiple comparisons test). (d) Time course of IL-12p40 production induced by Z-100 (100 μg/mL). Symbols indicate a significant difference compared with the saline group; (repeated measures ANOVA). (e, f) TNF-α (e) and IL-1β (f) concentration in the culture supernatant of BMDM after stimulation for 24 hours with Z-100 (100 μg/mL). Symbols indicate a significant difference compared with the saline group; (Student’s -test). Data show the ; per group, pooled from 3 experiments.

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