Peptidoglycan-Like Components in Z-100, Extracted from Mycobacterium tuberculosis Strain Aoyama B, Increase IL-12p40 via NOD2

Figure 2

Cytokine production from human CD14⁺ cells after stimulation with Z-100. (a) Schematic of the experimental design. Human CD14⁺ cells were cultured with GM-CSF for 7 days before being stimulated for 24 hours. (b–f) Cytokine concentration in the culture supernatant of human CD14⁺ cells after stimulation with Z-100 (b, d, f; 1, 10, or 100 μg/mL) or Pam3CSK4 (c, e; 3, 10, or 30 ng/mL). Symbols indicate a significant difference compared with the 0 μg/mL (saline) group; , (Steel’s multiple comparisons test). Data show the ; per group, pooled from 3 experiments.

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