Cecal Patches Generate Abundant IgG2b-Bearing B Cells That Are Reactive to Commensal Microbiota

Figure 1

The role of gut microbiota in GC formation and antibody expression on B cells in the GALT of the small and large intestines. Flow cytometric analysis of mononuclear cells of GALT in CV and GF mice was performed. (a) Left panel: flow cytometric dot plot of CD95 and GL7 expression in B220⁺ cells. Right panel: percentage and absolute numbers of CD95⁺GL7⁺B220⁺ GC B cells and CD95⁺GL7^-/intB220⁺ pre-GC B cells. (b) Left panel: flow cytometric dot plot of CXCR5 and PD-1 expression in CD4⁺CD3⁺ cells. Right panel: percentage and absolute numbers of CXCR5⁺PD-1⁺CD4⁺CD3⁺ Tfh cells. (c) The percentage of B220⁺ total B cells, CD95⁺GL7⁺B220⁺ GC B cells, and CD95⁺GL7^-/intB220⁺ pre-GC B cells expressing IgA or IgG2b. Data are shown as the and are representative of flow cytometric dot plots from six to eight independent experiments. , , and .

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