Cecal Patches Generate Abundant IgG2b-Bearing B Cells That Are Reactive to Commensal Microbiota

Figure 3

The role of MyD88 signaling in GC formation and antibody expression on B cells in GALT from the small and large intestines. Flow cytometric analysis of the mononuclear cells of each GALT from SPF WT and MyD88^-/- mice was performed. (a) Left panel: flow cytometric dot plot of CD95 and GL7 expression in B220⁺ cells. Right panel: percentage and absolute numbers of CD95⁺GL7⁺B220⁺ GC B cells and CD95⁺GL7^-/intB220⁺ pre-GC B cells. (b) Left panel: flow cytometric dot plot of CXCR5 and PD-1 expression in CD4⁺CD3⁺ cells. Right panel: percentage and absolute numbers of CXCR5⁺PD-1⁺CD4⁺CD3⁺ Tfh cells. (c) The percentage of B220⁺ total B cells, CD95⁺GL7⁺B220⁺ GC B cells, and CD95⁺GL7^-/intB220⁺ pre-GC B cells expressing IgA or IgG2b. Data are shown as and are representative of the flow cytometric dot plot from seven to eight independent experiments. , , and .

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