Cecal Patches Generate Abundant IgG2b-Bearing B Cells That Are Reactive to Commensal Microbiota

Figure 4

Production of the different antibody isotypes by GALT cells from small and large intestines. Mononuclear cells from GALT in CV BALB/c mice were stimulated in vitro with 1 μg/ml LPS, 1 μg/ml Pam3CSK4, or 1 μM of CpG DNA for 7 days. (a, b) The concentrations of total IgA and IgG2b in the culture supernatants were measured by ELISA. Data are shown as . (c, d) The levels of fecal bacteria-reactive IgA and IgG2b in the culture supernatants were measured by ELISA. Antibody titers of fecal bacteria-reactive antibodies are shown as net OD405 units (). The mean OD405 value of blanks was 0.21 for IgA and 0.07 for IgG2b. The results are representative of at least three independent experiments. , , and .

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