Cecal Patches Generate Abundant IgG2b-Bearing B Cells That Are Reactive to Commensal Microbiota

Figure 5

Levels of total and commensal microbiota-reactive antibodies in the feces and serum of the mice maintained under the different experimental conditions. The levels of total or commensal microbiota-reactive antibodies were measured by ELISA. (a) Total IgA in the fecal extract from CV and GF WT mice. (b) Total IgA, IgG2b, and IgM in serum from CV and GF WT mice. (c) Levels of total IgA in the fecal extract from SPF WT and MyD88^-/- mice. (d) Total IgA, IgG2b, and IgM in serum from SPF WT and MyD88^-/- mice. (e) Commensal microbiota-reactive IgA and IgG2b from CV and GF WT mice. (f) Commensal microbiota-reactive IgA and IgG2b from SPF WT and MyD88^-/- mice. (e, f) Antibody titers of commensal microbiota-reactive antibodies are shown as net OD405 units (). The mean OD405 value of blanks was 0.13 for IgA and 0.06 for IgG2b. Data are shown as the of six to ten mice and are representative of at least three independent experiments. , , and .

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