Epigenetic Regulation of IFI44L Expression in Monocytes Affects the Functions of Monocyte-Derived Dendritic Cells in Systemic Lupus Erythematosus

Figure 3

pSTAT3 recruits TET2 inducing DNA demethylation of IFI44L promoter in SLE monocyte. (a) Recruitment of TET2 by IFN-α-activated pSTAT3 in CD14⁺ monocytes was detected with Co-IP. Column 1 showed the expression of TET2 recruited by pSTAT3 with IFN-α treated. Column 2 showed the expression of TET2 recruited by pSTAT3 without IFN-α treated. Column 3 is negative control. (b) RT-qPCR analysis of TET1, TET2, and TET3 mRNA expression levels in CD14⁺ monocytes of SLE patients and healthy controls. (c, d) DNA methylation levels of two CpG sites at the IFI44L promoter in STAT3 overexpression and control groups were detected with BSP. ; ns: not significant. Data are shown as . Student’s -test was used to compare the results.

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