Research Article

Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p

Figure 4

Inhibition of 5-lipoxygenase (5-LO) attenuates the promotive effects of NS1 on IL-5 but had no effect on miR-19a-3p. (a) A549 cells were treated with MK886 of different concentrations, and the cell viability was assessed after 4 hours using cell counting kit-8 assay. Data are reported as the percentage relative to 0 μM. (b) Western blot analysis of 5-LO, IL-5, and NS1 protein levels in A549 cells transfected with pNS1 four hours after transfection. (c) Relative quantitative analysis of 5-LO protein and IL-5 protein, respectively. β-Actin served as an internal control. (d–f) The mRNA expression levels of miR-19a-3p, IL-5, and 5-LO in A549 cells transfected with pNS1 were detected by RT-qPCR four hours after transfection. U6 RNA or GADPH was used as internal control, respectively. Data are expressed as ( technical replicates). The experiment was performed three times with similar results, and one representative experiment is shown. vs. control group. NS1: nonstructural protein 1; pNS1: nonstructural protein 1 expressing plasmid; 5-LO: 5-lipoxygenase; IL-5: interleukin-5.
(a)
(b)
(c)
(d)
(e)
(f)