Human Neutrophil Antigen Genotype and Allele Frequencies in Iranian Blood Donors

Figure 2

Flow cytometry analysis of HNA-2 expression on the neutrophils. The neutrophils were stained in whole blood with FITC conjugated anti-CD177 antibody. (a, b) Unstained and isotype controls were used to set up the flow cytometer and analyze the results. Based on HNA-2 expression, two distinct populations were identified. Among donors, the expression of the HNA-2 antigens was varied. For analysis, individuals were divided into four categories: (c) HNA-2 expression between 5% and 50%, (d) HNA-2 expression between 50% and 60%, (e) HNA-2 expression more than 60%, and (f) with the HNA-2 expression less than 5% (negative). (g) Individuals with two positive populations were analyzed as atypical expression of the HNA-2 antigen. In the histogram plots, CD177 positive and negative neutrophils were identified using bifurcated gates.

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