Human Neutrophil Antigen Genotype and Allele Frequencies in Iranian Blood Donors

Table 2

List of primer sequences for HNA genotyping using PCR-SSP.

HNA antigens	Primer sequence 5 → 3	Amplification size (based Paris)	References
HNA-1a	HNA1a F CAATGGTACAGGGTGCTC	115	[13]
	HNA1a R CCTGGCTTGAGATGAGGT
HNA-1b	HNA1b F CCTCAATGGTACAGCGTGCTT	159	[13]
	HNA1b R ACTGTCGTTGACTGTGTCAG
HNA-1c	HNA1c F CCTCAATGGTACAGCGTGCTT	160	[13]
	HNA1c R CACTGTCGTTGACTGTGTCAT
HNA-3a	HNA3a F AGT GGC TGA GGT GCT TCG	291
	HNA3a R GTG CGC CAA TAT CCT CAC TTG
HNA-3b	HNA3b F GAGTGGCTGAGGTGCTTCA	292
	HNA3b R GTG CGC CAA TAT CCT CAC TTG
HNA-4a	HNA4a F TCA TGC GAG CCC ATC CG	248
	HNA4a R ACA AGG AGG TCT GAC GGT G
HNA-4b	HNA4b F CTC ATG CGA GCC CAT CCA	249
	HNA4b R ACA AGG AGG TCT GAC GGT G
HNA-5a	HNA5a F TTCTGATATTCCCCACCCTG	82	[13]
	HNA5a R CAGTTAGACGCAGGGCTC
HNA-5b	HNA5b F TTCTGATATTCCCCACCCTG	84	[13]
	HNA5b R AGCAGTTAGACGCAGGGCTG
Internal control	HGHF CAG TGC CTT CCCAACCATTCCCTTA	495	[13]
	HGHR ATC CAC TCA CGG ATT TCT GTT GTG TTT C

HNA: human neutrophil antigen; F: forward; R: reverse; HGH: human growth hormone. The primer sequences were obtained from the ISBT reference granulocyte laboratory in Giessen, Germany.