ZNF165 Is Involved in the Regulation of Immune Microenvironment and Promoting the Proliferation and Migration of Hepatocellular Carcinoma by AhR/CYP1A1

Figure 4

ZNF165 regulates AhR transcriptional activity. (a) ZNF165 overexpression significantly facilitated the luciferase activity of pGL3-AhR promoter, similar to TCDD a positive control of inducing AhR nuclear translocation. (b) The luciferase activity of the pGL3-AhR promoter was significantly inhibited by ZNF165 knockdown. (c) The overexpression of ZNF165 increased, while the knockdown of ZNF165 reduced AhR nuclear translocation. As a further confirmation, the nuclear and cytoplasm levels of AhR protein were examined in shZNF165-1/2- or OE-ZNF165-transduced cells. (d) After ZNF165 overexpression, AhR levels were significantly increased in the nucleus and decreased in the cytoplasm. After knocking down of ZNF165, AhR levels were significantly decreased in the nucleus and increased in the cytoplasm.

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