Anticancer Effect of Puerarin on Ovarian Cancer Progression Contributes to the Tumor Suppressor Gene Expression and Gut Microbiota Modulation

<div>Puerarin treatment activates cell apoptosis and related proteins, while suppressing IL-6, TNF-<i>α</i>, and CA125 levels and modulating CD3+CD4+CD3+/CD8+ T lymphocyte percentages in OC tumor-bearing Fischer 344 rats. (a) TUNEL staining for the detection of cell apoptosis in OC tumor tissues (magnification: ×200). (b) Protein expression levels of Bax, Bcl-2, cleaved caspase 3, and caspase 3 in tumor tissues were detected by Western blot assay, and GAPDH was used as the internal control. (c) Serum levels of IL-6, TNF-<i>α</i>, and CA125 were detected by ELISA. (d) Flow cytometry was used to detect the CD3+CD4+CD3+/CD8+ T lymphocyte percentages. The data are presented as <span class="nowrap"><svg height="9.10327pt" id="M22" style="vertical-align:-0.3499298pt" version="1.1" viewbox="-0.0498162 -8.75334 58.1969 9.10327" width="58.1969pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M797 0V26C739 32 732 36 732 103V296C732 394 682 449 605 449C576 449 550 437 529 423C504 407 475 389 446 366C425 418 382 449 334 449C303 449 279 437 253 421C222 403 201 385 180 371V452C135 432 85 419 41 411V388C99 379 102 374 102 310V103C102 38 93 32 27 26V0H238V26C189 32 180 38 180 103V338C210 363 250 390 289 390C351 390 377 348 377 275V103C377 37 368 32 306 26V0H520V26C465 32 456 38 456 101V296C456 314 455 326 453 338C491 369 529 390 565 390C628 390 653 345 653 274V107C653 36 642 32 583 26V0H797Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.595,0)"><path d="M380 106C343 72 306 56 265 56C195 56 116 112 115 248C235 252 361 262 377 265C396 269 400 277 400 297C400 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238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,40.505,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,43.469,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,49.709,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>,</span> compared to the vehicle group. OC: ovarian cancer.</div>

Journal of Immunology Research

fig4

Figure 4

Figure 4: Anticancer Effect of Puerarin on Ovarian Cancer Progression Contributes to the Tumor Suppressor Gene Expression and Gut Microbiota Modulation 

Figure 4 | Anticancer Effect of Puerarin on Ovarian Cancer Progression Contributes to the Tumor Suppressor Gene Expression and Gut Microbiota Modulation 