Comparative Safety, Immunogenicity, and Efficacy of CEF Cell-Based and DF-1 Cell Line Adapted Infectious Bursal Disease Vaccines in Specific-Pathogen-Free Chickens

Figure 2

Agarose gel electrophoresis of PCR amplified products of IBDV LC–75 VP2 gene fragments (400 bp). M is the DNA ladder (100 bp, Fermentas), E₁ and E₂ is the RNase-free water (extraction control), P is the positive control (IBD vaccine seed D78 strain), and F is the free wells. 400 bp gene fragment of IBDV LC–75 adapted on DF-1 cells: passage 1 (Lane 3), passage 2 (Lane 4), and passage 3 (Lane 5); N is the negative control and Lane 6 is the gene fragment (400 bp) of IBDV LC–75 propagated on CEF cells.