Levels of Pathogenic Th17 and Th22 Cells in Patients with Rheumatoid Arthritis

Figure 1

Flow cytometry strategy for the analysis of Th17 and Th22 cells. (a) PBMC were labeled with MoAbs against CD4, CXCR3, IL-17, IL-22, CD243 (MDR-1), CD161, IFN-γ, and IL-10 and analyzed by multiparametric flow cytometry, as indicated. This analysis allowed to detect the cell subsets of interest, pathogenic Th17 cells, nonpathogenic Th17 cells, and Th22 lymphocytes. (b) Flow cytometry images of a representative analysis of a blood sample from a healthy donor are shown. The type of fluorochrome coupled to each antibody is indicated, and the negative stain controls were selected according to the fluorescence minus one (FMO) strategy.

(a)

(b)